The CDK4 Polyclonal Antibody (CAB16813) is a valuable tool for researchers studying CDK4, a key protein involved in cell cycle regulation and proliferation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to CDK4, this antibody allows for accurate detection and analysis of the protein in various cell types, making it an essential component in studies related to cell division, cancer, and other diseases.CDK4, a cyclin-dependent kinase, plays a crucial role in controlling the progression of cells through the G1 phase of the cell cycle. Dysregulation of CDK4 activity has been implicated in numerous diseases, including cancer, making it an attractive target for therapeutic interventions.
Research into the function and regulation of CDK4 is essential for developing novel treatment strategies that target cell cycle abnormalities in cancer cells.Overall, the CDK4 Polyclonal Antibody (CAB16813) is a reliable tool for researchers investigating the role of CDK4 in cell cycle control, cancer development, and other related processes. Its high specificity and sensitivity make it a valuable asset in advancing our understanding of cell cycle regulation and identifying potential therapeutic targets for disease intervention.
Product Name:
CDK4 Rabbit Polyclonal Antibody
SKU:
CAB16813
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CMM3; PSK-J3; K4
Positive Sample:
HeLa,MCF7,COS-7
Cellular Localization:
Cytoplasm, Membrane, Nucleus.
Calculated MW:
34kDa
Observed MW:
30kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Purification Method:
Affinity purification
Gene ID:
1019
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CDK4 knockout (KO) HeLa cells, using [KO Validated] CDK4 Rabbit pAb (CAB16813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
