CDK4 Polyclonal Antibody (CAB21317)
- SKU:
- CAB21317
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IP
- IF
Description
| Product Name: | CDK4 Polyclonal Antibody |
| SKU: | CAB21317 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-303 of human CDK4 (NP_000066.1). |
| Sequence: | VGCI FAEM FRRK PLFC GNSE ADQL GKIF DLIG LPPE DDWP RDVS LPRG AFPP RGPR PVQS VVPE MEES GAQL LLEM LTFN PHKR ISAF RALQ HSYL HKDE GNPE |
| Tested Applications: | WB IHC-P IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIF/ICC,1:50 - 1:200 |
| Synonyms: | CMM3; PSK-J3; K4 |
| Positive Sample: | HeLa,293T |
| Cellular Localization: | Cytoplasm, Membrane, Nucleus. |
| Calculated MW: | 34kDa |
| Observed MW: | 35kDa |
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
| Purification Method: | Affinity purification |
| Gene ID: | 1019 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (CAB21317) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
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