Anti-CDK11A Antibody (PACO31932)

Antibody Name:	CDK11A Antibody (PACO31932)
Antibody SKU:	PACO31932
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Cyclin-dependent kinase 11A protein (5-216AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot Positive WB detected in: PC-3 whole cell lysate, Rat kidney tissue All lanes: CDK11A antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 92, 91, 46, 19, 63, 50 kDa Observed band size: 92 kDa .
	Immunofluorescent analysis of Hela cells using PACO31932 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO31932 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Synonyms:

Cyclin-dependent kinase 11A (EC 2.7.11.22) (Cell division cycle 2-like protein kinase 2) (Cell division protein kinase 11A) (Galactosyltransferase-associated protein kinase p58/GTA) (PITSLRE serine/threonine-protein kinase CDC2L2), CDK11A, CDC2L2 CDC2L3 PITSLREB

UniProt Protein Function:	CDK11A: Appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression. The cleaved p110 isoform, p110C, binds to the serine/threonine kinase PAK1. The p58 isoform but not the p110 isoform or p110C interacts with CCND3. The p110 isoforms are found in large molecular weight complexes containing CCNL1 and SFRS7. The p58 isoform is specifically induced in G2/M phase of the cell cycle. Expressed ubiquitously. Some evidence of isoform-specific tissue distribution. Phosphorylation at Thr-436 or Tyr-437 inactivates the enzyme, while phosphorylation at Thr-583 activates it. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. 8 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, CMGC; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Kinase, protein; RNA processing; Cell cycle regulation; CMGC group; CDK family; PITSLRE subfamilyChromosomal Location of Human Ortholog: 1p36.33Cellular Component: nucleoplasm; cytoplasm; nucleusMolecular Function: protein serine/threonine kinase activity; cyclin-dependent protein kinase activity; ATP binding; protein kinase activityBiological Process: mitosis; regulation of transcription, DNA-dependent; regulation of cell cycle; apoptosis; regulation of cell growth; protein amino acid phosphorylation; regulation of mRNA processing
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the p34Cdc2 protein kinase family. p34Cdc2 kinase family members are known to be essential for eukaryotic cell cycle control. This gene is in close proximity to CDC2L1, a nearly identical gene in the same chromosomal region. The gene loci including this gene, CDC2L1, as well as metalloprotease MMP21/22, consist of two identical, tandemly linked genomic regions, which are thought to be a part of the larger region that has been duplicated. This gene and CDC2L1 were shown to be deleted or altered frequently in neuroblastoma with amplified MYCN genes. The protein kinase encoded by this gene could be cleaved by caspases and was demonstrated to play roles in cell apoptosis. Many transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of only two have been determined so far. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UQ88
NCBI GenInfo Identifier:	317373559
NCBI Gene ID:	728642
NCBI Accession:	Q9UQ88.4
UniProt Secondary Accession:	Q9UQ88,O95227, O95228, O96012, Q12821, Q12853, Q12854 Q2TAJ0, Q5QPR0, Q5QPR1
UniProt Related Accession:	Q9UQ88
Molecular Weight:	91,362 Da
NCBI Full Name:	Cyclin-dependent kinase 11A
NCBI Synonym Full Names:	cyclin-dependent kinase 11A
NCBI Official Symbol:	CDK11A
NCBI Official Synonym Symbols:	CDC2L2; CDC2L3; p58GTA; PITSLRE; CDK11-p46; CDK11-p58; CDK11-p110
NCBI Protein Information:	cyclin-dependent kinase 11A; PITSLRE B; PITSLRE protein kinase beta; cell division protein kinase 11A; cell division cycle 2-like protein kinase 2; PITSLRE serine/threonine-protein kinase CDC2L2; cell division cycle 2-like 2 (PITSLRE proteins); galactosyltransferase-associated protein kinase p58/GTA
UniProt Protein Name:	Cyclin-dependent kinase 11A
UniProt Synonym Protein Names:	Cell division cycle 2-like protein kinase 2; Cell division protein kinase 11A; Galactosyltransferase-associated protein kinase p58/GTA; PITSLRE serine/threonine-protein kinase CDC2L2
Protein Family:	Cyclin-dependent kinase
UniProt Gene Name:	CDK11A
UniProt Entry Name:	CD11A_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)