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Anti-CDK1 Antibody (CAB2438)

SKU:
CAB2438
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 1 Anti-CDK1 Antibody CAB2438
  • Cell Death Antibodies 1 Anti-CDK1 Antibody CAB2438
  • Cell Death Antibodies 1 Anti-CDK1 Antibody CAB2438
€139 - €419
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Description

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Anti-CDK1 Antibody (CAB2438)

The CDK1 Polyclonal Antibody (CAB2438) is a valuable tool for researchers studying CDK1, a key regulator of cell cycle progression and cell division. This antibody, generated in rabbits, is highly specific for human samples and is ideal for use in Western blot applications. By targeting the CDK1 protein, this antibody allows for accurate detection and analysis of CDK1 expression in various cell types.CDK1, also known as cyclin-dependent kinase 1, plays a pivotal role in cell cycle regulation by phosphorylating key proteins involved in controlling cell division. Dysregulation of CDK1 activity has been implicated in various diseases, including cancer, making it an attractive target for therapeutic interventions.

Research on CDK1 is essential for understanding the molecular mechanisms underlying cell cycle progression and identifying novel strategies for cancer treatment.Overall, the CDK1 Polyclonal Antibody (CAB2438) is a reliable tool for investigating the functions of CDK1 in cellular processes, providing valuable insights into the role of this protein in health and disease. It is a valuable resource for scientists conducting studies in cell biology, molecular biology, and cancer research.

Antibody Name:Anti-CDK1 Antibody
Antibody SKU:CAB2438
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, HeLa, THP-1, Rat spleen
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SEID QLFR IFRA LGTP NNEV WPEV ESLQ DYKN TFPK WKPG SLAS HVKN LDE
Gene ID:983
Uniprot:P06493
Cellular Location:Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Calculated MW:27kDa/34kDa
Observed MW:34kDa
Synonyms:CDK1, CDC2, CDC28A, P34CDC2
Background:The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; EC 2.7.11.23; EC 2.7.11.22; Motility/polarity/chemotaxis; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; centrosome; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; nucleus; cytosol

Molecular Function:RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: regulation of Schwann cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; histone phosphorylation; response to toxin; ventricular cardiac muscle cell development; regulation of embryonic development; centrosome cycle; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; response to activity; toll-like receptor 9 signaling pathway; response to amine stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; MAPKKK cascade; peptidyl-threonine phosphorylation; microtubule cytoskeleton organization and biogenesis; DNA repair; MyD88-dependent toll-like receptor signaling pathway; G1/S-specific transcription in mitotic cell cycle; peptidyl-serine phosphorylation; response to cadmium ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to copper ion; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication

NCBI Summary:The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:P06493
NCBI GenInfo Identifier:334302921
NCBI Gene ID:983
NCBI Accession:P06493.3
UniProt Secondary Accession:P06493,O60764, A8K7C4, C9J497,
UniProt Related Accession:P06493
Molecular Weight:297
NCBI Full Name:Cyclin-dependent kinase 1
NCBI Synonym Full Names:cyclin-dependent kinase 1
NCBI Official Symbol:CDK1  
NCBI Official Synonym Symbols:CDC2; CDC28A; P34CDC2  
NCBI Protein Information:cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
UniProt Protein Name:Cyclin-dependent kinase 1
UniProt Synonym Protein Names:Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
UniProt Gene Name:CDK1  
UniProt Entry Name:CDK1_HUMAN
Anti-CDK1 Antibody (CAB2438)Western blot analysis of extracts of various cell lines, using CDK1 antibody (CAB2438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-CDK1 Antibody (CAB2438)Immunohistochemistry of paraffin-embedded human esophagus using CDK1 antibody (CAB2438) at dilution of 1:100 (40x lens).
Anti-CDK1 Antibody (CAB2438)Immunohistochemistry of paraffin-embedded human gastric cancer using CDK1 antibody (CAB2438) at dilution of 1:100 (40x lens).

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