The CDH3 Polyclonal Antibody (CAB14235) is a valuable tool for research involving the CDH3 gene, which encodes for Cadherin-3, a calcium-dependent cell adhesion protein involved in various cellular processes including cell-cell adhesion, cell migration, and tumor suppression. This polyclonal antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CDH3 protein, researchers can effectively detect and analyze its expression in different cell types, making it an ideal choice for studies in cell biology, cancer research, and developmental biology.
Cadherin-3 is known to play a critical role in maintaining tissue integrity and organization, as well as regulating cell signaling pathways that influence cell proliferation and differentiation. Dysregulation of CDH3 expression has been implicated in various diseases, including cancer metastasis and developmental disorders. The CDH3 Polyclonal Antibody (CAB14235) provides researchers with a reliable tool to investigate the role of Cadherin-3 in these pathological conditions, ultimately contributing to the development of targeted therapies and diagnostic tools.
Product Name:
CDH3 Rabbit Polyclonal Antibody
SKU:
CAB14235
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-350 of human CDH3 (NP_001784.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
91kDa
Observed MW:
115kDa
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS).
Purification Method:
Affinity purification
Gene ID:
1001
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDH3 Rabbit pAb (CAB14235) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
