The CDCA7 Polyclonal Antibody (PACO22028) is a powerful tool for researchers studying CDCA7, a key protein involved in cell division and proliferation. This antibody, produced in rabbits, demonstrates high specificity and sensitivity in recognizing CDCA7 in human samples, making it ideal for use in Western blot applications. By binding to CDCA7, this antibody enables precise detection and analysis of the protein in various cell types.CDCA7, also known as Cell division cycle-associated protein 7, plays a crucial role in the regulation of cell cycle progression and DNA replication.
Its involvement in these fundamental cellular processes highlights its importance in cancer research, as dysregulation of CDCA7 is often linked to uncontrolled cell growth and tumor formation. By targeting CDCA7 with this antibody, researchers can gain valuable insights into the mechanisms underlying oncogenesis and potentially identify new therapeutic strategies for cancer treatment.
Antibody Name:
CDCA7 Antibody (PACO22028)
Antibody SKU:
PACO22028
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human CDCA7.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from K562 cells, using CDCA7 antibody.
Background:
Participates in MYC-mediated cell transformation and apoptosis; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator.
Synonyms:
Cell division cycle-associated protein 7; Protein JPO1; JPO1;
UniProt Protein Function:
CDCA7: Participates in MYC-mediated cell transformation; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 2q31Cellular Component: nucleoplasm; cytoplasm; nucleusBiological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; regulation of cell proliferation
UniProt Protein Details:
NCBI Summary:
This gene was identified as a c-Myc responsive gene, and behaves as a direct c-Myc target gene. Overexpression of this gene is found to enhance the transformation of lymphoblastoid cells, and it complements a transformation-defective Myc Box II mutant, suggesting its involvement in c-Myc-mediated cell transformation. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
