The CDC6 Rabbit Polyclonal Antibody (CAB18249) is a valuable tool for researchers studying CDC6, a key regulator of DNA replication and cell cycle progression. This antibody, produced in rabbits, is highly specific and reliable for detecting CDC6 protein in various samples, particularly human tissues. Validated for use in Western blot applications, the antibody binds to CDC6 with high affinity, allowing for accurate analysis and quantification in research settings.CDC6 is essential for the initiation of DNA replication and is tightly regulated during the cell cycle to ensure proper DNA synthesis and cell division.
Dysregulation of CDC6 has been implicated in various diseases, including cancer, making it an important target for therapeutic intervention. By studying CDC6 expression and activity using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying cell proliferation and potential therapeutic strategies for cancer treatment.
Product Name:
Cdc6 Rabbit Polyclonal Antibody
SKU:
CAB18249
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-560 of human Cdc6 (Q99741).
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Purification Method:
Affinity purification
Gene ID:
990
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Cdc6 Rabbit pAb (CAB18249) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
