The CDC45 Polyclonal Antibody (CAB2047) is a valuable tool for research into CDC45, a key protein involved in DNA replication and cell cycle progression. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CDC45 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cancer research.
CDC45 is essential for the initiation and progression of DNA replication, making it a crucial target for understanding cellular proliferation and growth. Dysregulation of CDC45 has been linked to various diseases, including cancer, making it an attractive focus for therapeutic intervention. Research into the function and regulation of CDC45 is vital for developing strategies to target DNA replication processes in cancer cells and other disease states.
Product Name:
CDC45 Rabbit Polyclonal Antibody
SKU:
CAB2047
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_003495.1).
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8318
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using cdc45 antibody (CAB2047) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
