The CDC42EP4 Polyclonal Antibody (PACO22246) is a crucial tool for researchers studying the CDC42EP4 protein, a key regulator of cell migration and invasion. This antibody, derived from rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the CDC42EP4 protein, this antibody enables researchers to detect and analyze its expression levels in various cell types, facilitating investigations in the fields of cell biology and cancer research.
CDC42EP4, also known as CDC42 effector protein 4, plays a pivotal role in regulating the activity of the CDC42 protein, a key player in cell signaling pathways involved in cytoskeletal organization and cell movement. Understanding the function of CDC42EP4 is essential for elucidating its role in cancer metastasis and other pathological processes where cell migration is dysregulated. By targeting CDC42EP4, researchers can develop novel therapeutic strategies aimed at inhibiting cancer cell invasion and metastasis.
Antibody Name:
CDC42EP4 Antibody (PACO22246)
Antibody SKU:
PACO22246
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human BORG4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using BORG4 antibody.
Background:
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation, when overexpressed in fibroblasts.
Synonyms:
binder of Rho GTPases 4; BORG4; CDC42 effector protein (Rho GTPase binding) 4; Cdc42 effector protein 4; CEP4
UniProt Protein Function:
CDC42EP4: Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation, when overexpressed in fibroblasts. Belongs to the BORG/CEP family.Protein type: CytoskeletalChromosomal Location of Human Ortholog: 17q24-q25Cellular Component: microtubule cytoskeleton; cytoplasm; plasma membrane; endomembrane system; actin cytoskeletonMolecular Function: GTP-Rho bindingBiological Process: regulation of cell shape; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; Rho protein signal transduction
UniProt Protein Details:
NCBI Summary:
The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq, Jul 2008]
