The CDC42EP3 Polyclonal Antibody (CAB8480) is a valuable tool for researchers studying CDC42EP3, a protein involved in cell signaling pathways and cytoskeletal organization. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the CDC42EP3 protein, this antibody allows for the detection and analysis of CDC42EP3 expression in a variety of cell types, making it ideal for studies in cell biology and cancer research.
CDC42EP3, also known as CDC42 effector protein 3, regulates the activity of the small GTPase CDC42, which plays a crucial role in cell migration, proliferation, and differentiation. Abnormalities in CDC42EP3 expression have been linked to cancer progression and metastasis, making it a potential target for therapeutic interventions. Research on CDC42EP3 can provide valuable insights into the mechanisms of cell signaling and potential therapeutic strategies for cancer treatment.
Product Name:
CDC42EP3 Rabbit Polyclonal Antibody
SKU:
CAB8480
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human CDC42EP3 (NP_006440.2).
This gene encodes a member of a small family of guanosine triphosphate (GTP) metabolizing proteins that contain a CRIB (Cdc42, Rac interactive binding) domain. Members of this family of proteins act as effectors of CDC42 function. The encoded protein is involved in actin cytoskeleton re-organization during cell shape changes, including pseudopodia formation. A pseudogene of this gene is found on chromosome 19. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10602
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDC42EP3 antibody (CAB8480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
