The CDC42BPB Antibody (PACO57052) is a polyclonal antibody designed for research involving CDC42BPB, a protein known for its role in regulating cell signaling pathways. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various experimental applications, including Western blotting.CDC42BPB, also known as CDC42-binding protein kinase beta, is an essential regulator of cell growth, differentiation, and apoptosis. It plays a crucial role in mediating cellular responses to extracellular stimuli and is involved in processes such as cytoskeletal reorganization and cell migration.
Understanding the function of CDC42BPB is key to unraveling its implications in various diseases, including cancer and neurodegenerative disorders.Researchers studying cell signaling pathways, protein-protein interactions, and kinase activity will find the CDC42BPB Antibody (PACO57052) to be a valuable tool in their experiments. Its specificity and sensitivity make it ideal for detecting and analyzing CDC42BPB in a variety of cell types, providing insights into the mechanisms underlying cellular processes and disease development.
IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO57052 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.
MRCKB: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; GEK subfamilyChromosomal Location of Human Ortholog: 14q32.3Cellular Component: actomyosin; cytoplasm; cytoskeleton; intercellular junction; leading edge; plasma membraneMolecular Function: ATP binding; magnesium ion binding; protein kinase activity; protein serine/threonine kinase activity; Rho GTPase bindingBiological Process: actin cytoskeleton reorganization; actomyosin structure organization and biogenesis; cell migration; cytoskeleton organization and biogenesis; establishment and/or maintenance of cell polarity; protein amino acid phosphorylation; signal transduction
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
