The CDC42BPB Polyclonal Antibody (PAC023300) is a valuable tool for researchers studying CDC42BPB, a protein involved in cell signaling and regulation. This antibody is produced in rabbits and has high reactivity with human samples, making it ideal for Western blot applications. By binding to the CDC42BPB protein, this antibody enables accurate detection and analysis of CDC42BPB in various cell types.CDC42BPB is known to play a role in cellular processes such as cell division, migration, and differentiation, making it a key player in pathways related to cancer development and progression.
Research into CDC42BPB's functions and interactions can provide valuable insights into the mechanisms underlying cancer and other diseases. This antibody is a valuable tool for investigating the role of CDC42BPB in various physiological and pathological processes, making it an indispensable resource for studies in cell biology and oncology.
Synthesized peptide derived from internal of human MRCKB.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells, using MRCKB antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using MRCKB antibody.
Background:
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.
Synonyms:
CDC42-binding protein kinase beta; CDC42BPB; EC 2.7.11.1; MRCK-beta;
UniProt Protein Function:
MRCKB: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; GEK subfamilyChromosomal Location of Human Ortholog: 14q32.3Cellular Component: actomyosin; cytoplasm; cytoskeleton; intercellular junction; leading edge; plasma membraneMolecular Function: ATP binding; magnesium ion binding; protein kinase activity; protein serine/threonine kinase activity; Rho GTPase bindingBiological Process: actin cytoskeleton reorganization; actomyosin structure organization and biogenesis; cell migration; cytoskeleton organization and biogenesis; establishment and/or maintenance of cell polarity; protein amino acid phosphorylation; signal transduction
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
