The CDC42 Polyclonal Antibody (CAB1188) is a valuable tool for researchers studying CDC42, a small GTPase protein that regulates various cellular functions such as cell cycle progression, cell migration, and cell polarity. The antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.CDC42 is known for its involvement in signaling pathways that control cytoskeletal organization, cell adhesion, and membrane trafficking, making it a key player in processes such as cell division and cell motility.
Dysregulation of CDC42 activity has been linked to a variety of diseases, including cancer, neurological disorders, and immune system dysfunction.By specifically targeting the CDC42 protein, this antibody enables researchers to detect and analyze CDC42 expression levels in different cell types, providing valuable insights into its role in cellular processes and disease pathogenesis. Its versatility and reliability make it an essential tool for studies in cell biology, cancer research, and drug development aimed at targeting CDC42 for therapeutic interventions.
Product Name:
CDC42 Rabbit Polyclonal Antibody
SKU:
CAB1188
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 118-191 of human CDC42 (NP_001782.1).
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
Purification Method:
Affinity purification
Gene ID:
998
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDC42 Rabbit pAb (CAB1188) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
