The CDC37 Polyclonal Antibody (CAB2859) is specifically designed for research related to CDC37, a co-chaperone essential for the stability and function of protein kinases. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the CDC37 protein, this antibody allows for precise detection and analysis in various cell types, making it an ideal tool for studies in cell biology and cancer research.CDC37 is a crucial regulator of protein kinase activity, playing a key role in the folding and maturation of these important signaling molecules.
Its involvement in the stability and function of protein kinases implicates CDC37 in various cellular processes, making it an attractive target for research into cancer and other diseases characterized by dysregulated protein kinase signaling pathways. Understanding the role of CDC37 is essential for developing targeted therapies that exploit the vulnerabilities of cancer cells and other diseases driven by aberrant protein kinase activity.
Product Name:
CDC37 Rabbit Polyclonal Antibody
SKU:
CAB2859
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human CDC37 (NP_008996.1).
WB,1:200 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
P50CDC37; CDC37
Positive Sample:
Raji,HeLa,HepG2,BT474,HT-1080
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
50kDa
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Purification Method:
Affinity purification
Gene ID:
11140
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDC37 Rabbit pAb (CAB2859) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
