The CDC27 Polyclonal Antibody (CAB1954) is a valuable tool for researchers studying the CDC27 protein, a key component of the anaphase-promoting complex/cyclosome (APC/C) that regulates cell cycle progression. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications.CDC27 plays a critical role in controlling cell division and ensuring accurate segregation of chromosomes during mitosis. Dysregulation of CDC27 expression or function has been associated with various diseases, including cancer, making it an important target for further investigation.
By utilizing the CDC27 Polyclonal Antibody, researchers can effectively study the expression and localization of CDC27 in different cell types, advancing our understanding of its role in cell cycle regulation and potential implications for disease development.Overall, the CDC27 Polyclonal Antibody is a reliable tool for researchers in the fields of cell biology, oncology, and molecular biology who are interested in unraveling the mechanisms underlying cell division and identifying novel therapeutic targets for cancer treatment.
Product Name:
CDC27 Rabbit Polyclonal Antibody
SKU:
CAB1954
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1).
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
Purification Method:
Affinity purification
Gene ID:
996
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using CDC27 Rabbit pAb (CAB1954) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).
