CDC27 Rabbit Polyclonal Antibody (CAB1954)
- SKU:
- CAB1954
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
Product Name: | CDC27 Rabbit Polyclonal Antibody |
SKU: | CAB1954 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1). |
Sequence: | HLQK DVAL SVLS KDLT DMDK NSPE AWCA AGNC FSLQ REHD IAIK FFQR AIQV DPNY AYAY TLLG HEFV LTEE LDKA LACF RNAI RVNP RHYN AWYG LGMI YYKQ EKFS LAEM HFQK ALDI NPQS SVLL CHIG VVQH ALKK SEKA LDTL NKAI VIDP KNPL CKFH RASV LFAN EKYK SALQ ELEE LKQI VPKE SLVY FLIG KVYK KLGQ THLA LMNF SWAM DLDP KGAN NQIK EAID KRYL PDDE EPIT QEEQ IMGT DESQ ESSM TDAD DTQL HAAE SDEF |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E; CDC27 |
Positive Sample: | HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 92kDa |
Observed MW: | 110kDa |
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
Purification Method: | Affinity purification |
Gene ID: | 996 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |