The CDC25B Polyclonal Antibody (CAB9758) is a valuable tool for researchers studying the CDC25B protein, a phosphatase involved in cell cycle regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to CDC25B, researchers can accurately detect and analyze this protein in a variety of cell types, making it ideal for investigations in cell biology and cancer research.CDC25B is a key player in cell cycle progression, promoting cell division by activating cyclin-dependent kinases.
Dysregulation of CDC25B has been implicated in various types of cancer, making it an attractive target for therapeutic intervention. Understanding the role of CDC25B in cell cycle control is essential for developing novel treatments for cancer and other proliferative disorders.Overall, the CDC25B Polyclonal Antibody (CAB9758) offers researchers a reliable tool for studying the function of CDC25B in health and disease, providing insights that may lead to innovative therapeutic strategies in the future.
Product Name:
CDC25B Rabbit Monoclonal Antibody
SKU:
CAB9758
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CDC25B (P30305).
Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
65kDa
Observed MW:
60kDa
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
Purification Method:
Affinity purification
Gene ID:
994
Clone Number:
ARC1736
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDC25B Rabbit mAb (CAB9758) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
