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CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00263
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Cycle
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€599
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Description

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CDC25A (Phospho-Ser178)Colorimetric Cell-Based ELISA Kit

The CDC25A Phospho-Ser178 Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for accurately measuring levels of CDC25A phosphorylated at serine 178 in cell lysates and tissue extracts. This kit offers exceptional sensitivity and specificity, delivering reliable and reproducible results for a variety of research applications.CDC25A is a key regulatory protein involved in cell cycle progression, specifically the control of cell division. Phosphorylation of CDC25A at serine 178 is critical for its function and dysregulation of this process has been linked to various diseases including cancer and neurodegenerative disorders.

Therefore, monitoring the phosphorylation status of CDC25A can provide valuable insights into these conditions and potential therapeutic strategies.The CDC25A Phospho-Ser178 Colorimetric Cell-Based ELISA Kit is a valuable tool for researchers studying cell cycle regulation, cancer biology, and other related fields. Its high performance and ease of use make it an essential component for any laboratory conducting cutting-edge research in these areas.

Product Name:CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA
Product Code:CBCAB00263
ELISA Type:Cell-Based
Target:CDC25A (Phospho-Ser178)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect CDC25A protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CDC25A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDC25A phosphorylation.

Qualitative determination of CDC25A (Phospho-Ser178) concentration is achieved by an indirect ELISA format. In essence, CDC25A (Phospho-Ser178) is captured by CDC25A (Phospho-Ser178)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 993, UniProt ID: P30304, OMIM: 116947, Unigene: Hs.437705
Gene Symbol:CDC25A
Sub Type:Phospho
UniProt Protein Function:CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.
UniProt Protein Details:

Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function:protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle

NCBI Summary:CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P30304
NCBI GenInfo Identifier:50403734
NCBI Gene ID:993
NCBI Accession:P30304.2
UniProt Secondary Accession:P30304,Q8IZH5, Q96IL3, Q9H2F2,
UniProt Related Accession:P30304
Molecular Weight:
NCBI Full Name:M-phase inducer phosphatase 1
NCBI Synonym Full Names:cell division cycle 25A
NCBI Official Symbol:CDC25A  
NCBI Official Synonym Symbols:CDC25A2  
NCBI Protein Information:M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A
UniProt Protein Name:M-phase inducer phosphatase 1
UniProt Synonym Protein Names:Dual specificity phosphatase Cdc25A
Protein Family:M-phase inducer phosphatase
UniProt Gene Name:CDC25A  
UniProt Entry Name:MPIP1_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-CDC25A (Phospho-Ser178) Antibody, Anti-CDC25A Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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