The CDC23 Polyclonal Antibody (CAB15116) is a valuable tool for researchers studying the CDC23 protein, a crucial component of the anaphase-promoting complex/cyclosome (APC/C) involved in cell cycle regulation. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By targeting the CDC23 protein, researchers can detect and analyze its expression in various cell types, aiding in studies related to cell division, proliferation, and cancer research.
CDC23 is essential for the proper functioning of the APC/C complex, which regulates the progression through the cell cycle by triggering the degradation of specific proteins. Dysregulation of this complex can lead to cell cycle abnormalities and potentially contribute to the development of cancer. Understanding the role of CDC23 in these processes may provide insights into potential therapeutic targets for cancer treatment and other cell cycle-related disorders.
Product Name:
CDC23 Rabbit Polyclonal Antibody
SKU:
CAB15116
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
Purification Method:
Affinity purification
Gene ID:
8697
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDC23 Rabbit pAb (CAB15116) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
