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CD95 monoclonal antibody (APC) (AGIM0486)

SKU:
AGIM0486
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG1
Clone:
LT95
Conjugation:
APC
Applications:
FC
€179
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Description

system_update_altDatasheet
Product Name:CD95 monoclonal antibody (APC)
Product Code:AGIM0486
Size:100 µL
Reactivity:Human
Clone:LT95
Applications:Flow Cytometry
Conjugate:APC
Isotype:IgG1
Host Species:Mouse
Storage:Store at 4°C. Avoid prolonged exposure to light.
Uniprot:P25445
Background:CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
UniProt Protein Function:FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Membrane protein, integral; Receptor, cytokine; Cell surface

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: cell surface; cytoplasm; plasma membrane; integral to membrane; CD95 death-inducing signaling complex; nucleus; cytosol; lipid raft; external side of plasma membrane

Molecular Function:identical protein binding; protein binding; signal transducer activity; transmembrane receptor activity; receptor activity; kinase binding

Biological Process: circadian rhythm; caspase activation; spleen development; regulation of myeloid cell differentiation; renal system process; transformed cell apoptosis; apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization; regulation of lymphocyte differentiation; response to toxin; response to glucocorticoid stimulus; negative regulation of B cell activation; signal transduction; negative thymic T cell selection; regulation of apoptosis; inflammatory cell apoptosis; B cell mediated immunity; induction of apoptosis via death domain receptors; gene expression; protein complex assembly; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; negative regulation of apoptosis

Disease: Autoimmune Lymphoproliferative Syndrome

NCBI Summary:The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
UniProt Code:P25445
NCBI GenInfo Identifier:119833
NCBI Gene ID:355
NCBI Accession:P25445.1
UniProt Secondary Accession:P25445,Q14292, Q14293, Q14294, Q14295, Q16652, Q5T9P1 Q5T9P2, Q5T9P3, Q6SSE9, A9UJX4, B6VNV4,
UniProt Related Accession:P25445
Molecular Weight:37,732 Da
NCBI Full Name:Tumor necrosis factor receptor superfamily member 6
NCBI Synonym Full Names:Fas cell surface death receptor
NCBI Official Symbol:FAS  
NCBI Official Synonym Symbols:APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6  
NCBI Protein Information:tumor necrosis factor receptor superfamily member 6; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; FAS receptor variant 9; APO-1 cell surface antigen; TNF receptor superfamily member 6; apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); tumor necrosis factor receptor superfamily, member 6
UniProt Protein Name:Tumor necrosis factor receptor superfamily member 6
UniProt Synonym Protein Names:Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor
Protein Family:Development-specific protein
UniProt Gene Name:FAS  
UniProt Entry Name:TNR6_HUMAN

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