The CD86 Polyclonal Antibody (CAB16805) is a valuable tool for researchers studying CD86, a key cell surface molecule involved in immune activation and regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CD86 protein, this antibody enables precise detection and analysis of CD86 expression in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.
CD86, also known as B7-2, is a co-stimulatory molecule that plays a crucial role in T cell activation and the regulation of immune responses. Its involvement in modulating immune functions makes it a promising target for research into diseases such as cancer, autoimmune disorders, and inflammatory conditions. Understanding the function of CD86 is essential for the development of novel therapies aimed at manipulating the immune system in these pathological contexts.
Product Name:
CD86 Rabbit Polyclonal Antibody
SKU:
CAB16805
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-133 of human CD86 (NP_787058.4).
WB,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
B70; B7-2; B7.2; LAB72; CD28LG2; CD86
Positive Sample:
Raji,Mouse thymus,Rat thymus
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
38kDa
Observed MW:
65-70kDa
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
942
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD86 Rabbit pAb (CAB16805) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
