The CD86 Polyclonal Antibody (CAB1199) is a valuable tool for researchers studying CD86, a co-stimulatory molecule involved in immune activation. This antibody, generated from rabbit hosts, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the CD86 protein, this antibody enables reliable detection and analysis in a variety of cell types, making it an ideal choice for investigations in immunology and cancer research.CD86, also known as B7-2, plays a crucial role in T cell activation and immune response regulation. Its function as a co-stimulatory molecule allows CD86 to promote immune activation and cytokine production, leading to an enhanced immune response.
Research on CD86 is essential for understanding immune system dynamics and developing new therapies for diseases such as cancer, autoimmune disorders, and infectious diseases.Overall, the CD86 Polyclonal Antibody (CAB1199) is a valuable tool for researchers interested in studying immune activation and regulatory pathways involving the CD86 co-stimulatory molecule. Its high reactivity and specificity make it a reliable choice for Western blot applications and other research assays in the field of immunology.
Product Name:
CD86 Rabbit Polyclonal Antibody
SKU:
CAB1199
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-133 of human CD86 (NP_787058.4).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
38kDa
Observed MW:
60kDa
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
942
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Daudi cells, using CD86 Rabbit pAb (CAB1199) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
