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CD8 monoclonal antibody (APC ) (AGIM0457)

SKU:
AGIM0457
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG1, k
Clone:
HIT8a
Conjugation:
APC
Applications:
FC
€179
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Description

system_update_altDatasheet
Product Name:CD8 monoclonal antibody (APC )
Product Code:AGIM0457
Size:100 µL
Reactivity:Human
Clone:HIT8a
Applications:Flow Cytometry
Conjugate:APC
Isotype:IgG1, k
Host Species:Mouse
Storage:Store at 4°C. Avoid prolonged exposure to light.
Uniprot:P01732
Background:The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UniProt Protein Function:Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
UniProt Protein Details:

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in Disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

NCBI Summary:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:P01732
NCBI GenInfo Identifier:116035
NCBI Gene ID:925
NCBI Accession:P01732.1
UniProt Secondary Accession:P01732,Q13970, Q4ZG17, D6W5M8,
UniProt Related Accession:P01732
Molecular Weight:
NCBI Full Name:T-cell surface glycoprotein CD8 alpha chain
NCBI Synonym Full Names:CD8a molecule
NCBI Official Symbol:CD8A  
NCBI Official Synonym Symbols:CD8; MAL; p32; Leu2  
NCBI Protein Information:T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name:T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names:T-lymphocyte differentiation antigen T8/Leu-2
Protein Family:CD81 antigen
UniProt Gene Name:CD8A  
UniProt Entry Name:CD8A_HUMAN

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