The CD74 Rabbit Monoclonal Antibody (CAB9149) is a cutting-edge research tool designed for studying CD74, a transmembrane protein involved in antigen presentation and immune regulation. This high-quality antibody, produced in rabbits, exhibits excellent specificity and sensitivity for detecting CD74 in human samples. It has been rigorously validated for use in Western blot applications, enabling precise and reliable analysis of CD74 expression levels.CD74 is a key player in the immune response, serving as a chaperone protein for the MHC class II complex and facilitating antigen presentation to T cells.
Dysregulation of CD74 has been implicated in various diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. By utilizing the CD74 Rabbit Monoclonal Antibody, researchers can gain valuable insights into the role of CD74 in immune system function and disease pathology, paving the way for the development of novel treatment strategies.
Product Name:
CD74 Rabbit Monoclonal Antibody
SKU:
CAB9149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD74 (P04233).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome, Single-pass type II membrane protein, trans-Golgi network.
Calculated MW:
34kDa
Observed MW:
30-40kDa
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
972
Clone Number:
ARC1452
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD74 Rabbit mAb (CAB9149) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
