The CD74 Polyclonal Antibody (CAB5667) is a valuable tool for researchers studying the CD74 protein, a cell surface molecule involved in antigen presentation and immune regulation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is suitable for use in Western blot applications. By binding to the CD74 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for immunology and cancer research studies.CD74, also known as the HLA class II histocompatibility antigen gamma chain, plays a crucial role in the immune system by facilitating the presentation of antigens to T cells, thereby initiating immune responses.
Understanding the function and expression of CD74 is critical for investigating diseases such as cancer, autoimmune disorders, and inflammatory conditions. By elucidating the mechanisms by which CD74 contributes to immune responses, researchers can develop targeted therapies for these challenging conditions.
Product Name:
CD74 Rabbit Polyclonal Antibody
SKU:
CAB5667
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome, Single-pass type II membrane protein, trans-Golgi network.
Calculated MW:
34kDa
Observed MW:
30-40kDa
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
972
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Daudi cells, using CD74 Rabbit pAb (CAB5667) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
