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Anti-CD68 Antibody (CAB13286)

SKU:
CAB13286
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 3 Anti-CD68 Antibody CAB13286
  • Cell Biology Antibodies 3 Anti-CD68 Antibody CAB13286
  • Cell Biology Antibodies 3 Anti-CD68 Antibody CAB13286
  • Cell Biology Antibodies 3 Anti-CD68 Antibody CAB13286
  • Cell Biology Antibodies 3 Anti-CD68 Antibody CAB13286
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Description

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Anti-CD68 Antibody (CAB13286)

The CD68 Polyclonal Antibody (CAB13286) is a valuable tool for researchers studying CD68, a protein primarily found on the surface of macrophages and other phagocytic cells. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CD68 protein, it enables accurate detection and analysis in various cell types, making it ideal for investigations in immunology and cancer research.CD68 plays a crucial role in immune response and inflammation regulation, making it a key target for studies on diseases such as cancer, autoimmune disorders, and inflammatory conditions.

The antibody's ability to detect and analyze CD68 levels provides researchers with a powerful tool to explore its functions and potential therapeutic applications in these contexts. Overall, the CD68 Polyclonal Antibody is an essential resource for researchers seeking to deepen their understanding of immune regulation and disease pathogenesis.

Antibody Name:Anti-CD68 Antibody
Antibody SKU:CAB13286
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-319 of human CD68 (NP_001242.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, THP-1, U-937, RAW264.7, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-319 of human CD68 (NP_001242.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NDCP HKKS ATLL PSFT VTPT VTES TGTT SHRT TKSH KTTT HRTT TTGT TSHG PTTA THNP TTTS HGNV TVHP TSNS TATS QGPS TATH SPAT TSHG NATV HPTS NSTA TSPG FTSS AHPE PPPP SPSP SPTS KETI GDYT WTNG SQPC VHLQ AQIQ IRVM YTTQ GGGE AWGI SVLN PNKT KVQG SCEG AHPH LLLS FPYG HLSF GFMQ DLQQ KVVY LSYM AVEY NVSF PHAA QWTF SAQN ASLR DLQA PLGQ SFSC SNSS IILS PAVH LDLL SLRL QAAQ LPHT GVFG QSFS CPSD RS
Gene ID:968
Uniprot:P34810
Cellular Location:Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:31kDa/34kDa/37kDa
Observed MW:80-130KDa
Synonyms:CD68, GP110, LAMP4, SCARD1
Background:This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.
UniProt Protein Function:CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane

NCBI Summary:This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:P34810
NCBI GenInfo Identifier:147744552
NCBI Gene ID:968
NCBI Accession:P34810.2
UniProt Secondary Accession:P34810,Q53HR6, Q53XI3, Q96BI7, B4DVT4,
UniProt Related Accession:P34810
Molecular Weight:354
NCBI Full Name:Macrosialin
NCBI Synonym Full Names:CD68 molecule
NCBI Official Symbol:CD68  
NCBI Official Synonym Symbols:GP110; LAMP4; SCARD1  
NCBI Protein Information:macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
UniProt Protein Name:Macrosialin
UniProt Synonym Protein Names:Gp110; CD_antigen: CD68
Protein Family:Macrosialin
UniProt Gene Name:CD68  
UniProt Entry Name:CD68_HUMAN
Anti-CD68 Antibody (CAB13286)Western blot analysis of extracts of various cell lines, using CD68 antibody (CAB13286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-CD68 Antibody (CAB13286)Immunohistochemistry of paraffin-embedded Human oophoroma using CD68 antibody (CAB13286) at dilution of 1:100 (40x lens).
Anti-CD68 Antibody (CAB13286)Immunohistochemistry of paraffin-embedded Mouse spleen using CD68 antibody (CAB13286) at dilution of 1:100 (40x lens).
Anti-CD68 Antibody (CAB13286)Immunofluorescence analysis of THP-1 cells using CD68 antibody (CAB13286) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CD68 Antibody (CAB13286)Immunofluorescence analysis of Rat spleen using CD68 antibody (CAB13286) at dilution of 1:100. Blue: DAPI for nuclear staining.

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