The CD47 Polyclonal Antibody is a crucial tool for researchers studying CD47, a protein known for its role in immune regulation and its involvement in cancer development and progression. This high-quality antibody, generated in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.CD47, also known as integrin-associated protein, is a cell surface protein that plays a key role in modulating immune responses by preventing phagocytosis of cancer cells and regulating cell migration and adhesion.
Its dysregulation has been implicated in various diseases, including cancer, cardiovascular diseases, and autoimmune disorders.The CD47 Polyclonal Antibody binds specifically to the CD47 protein, allowing for accurate detection and analysis in a variety of cell types. This antibody is an invaluable tool for researchers in immunology, cancer biology, and other related fields, as it enables the investigation of CD47 function and its potential as a therapeutic target for diseases characterized by immune dysregulation.
Product Name:
CD47 Polyclonal Antibody
SKU:
CAB23052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant protein of mouse CD47.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
IAP; Itgp; 9130415E20Rik; B430305P08Rik; CD47
Positive Sample:
Mouse thymus,Rat spleen,Rat lung
Conjugate:
Unconjugated
Calculated MW:
33kDa
Observed MW:
45-60kDa
Predicted to enable protein binding activity involved in heterotypic cell-cell adhesion and thrombospondin receptor activity. Involved in regulation of nitric oxide biosynthetic process. Acts upstream of or within several processes, including monocyte aggregation; opsonization; and positive regulation of phagocytosis. Located in extracellular exosome. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human CD47 (CD47 molecule).
Purification Method:
Affinity purification
Gene ID:
16423
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Mouse thymus, using CD47 antibody (CAB23052) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
