The CD40 Monoclonal Antibody is a valuable tool for researchers studying the CD40 protein, a cell surface receptor involved in immune responses and inflammation. This antibody, produced through monoclonal technology, exhibits high specificity and sensitivity for human samples, making it an ideal choice for a variety of research applications, including Western blotting and immunohistochemistry.CD40 is a key player in the regulation of immune responses, acting as a co-stimulatory molecule that enhances the activation of immune cells such as B cells, dendritic cells, and macrophages.
Dysregulation of CD40 signaling has been implicated in various immune-related disorders, making it a promising target for therapeutic interventions.By utilizing the CD40 Monoclonal Antibody, researchers can effectively detect and analyze the CD40 protein in different cell types and tissues, shedding light on its role in immunity and inflammation. This antibody is a valuable tool for studies in immunology, oncology, and autoimmune diseases, providing valuable insights into the complex mechanisms underlying these conditions.
Product Name:
CD40 Monoclonal Antibody
SKU:
CAB22714
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human CD40.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
p50; Bp50; CDW40; TNFRSF5; CD40
Positive Sample:
U2OS,Daudi,Raji
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
31kDa
Observed MW:
40-45kDa
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
958
Clone Number:
ARC57627
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD40 antibody (CAB22714) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
