The CD40 Ligand Polyclonal Antibody (CAB0327) is a valuable tool for research involving the CD40 Ligand protein, which plays a crucial role in immune activation and cell signaling. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the CD40 Ligand protein, allowing for accurate detection and analysis in various cell types.CD40 Ligand, also known as CD154, is a key molecule involved in the regulation of immune responses, particularly in the activation of B cells and antigen-presenting cells. Dysregulation of the CD40 Ligand pathway has been implicated in various immune-related disorders, including autoimmune diseases and certain types of cancer.
By studying the function of CD40 Ligand, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new targets for therapeutic intervention.Overall, the CD40 Ligand Polyclonal Antibody is an essential tool for investigators studying immune responses, inflammation, and related pathways. Its high specificity and reliability make it an excellent choice for use in immunology, oncology, and other fields where understanding the role of CD40 Ligand is critical.
Product Name:
CD40L Rabbit Polyclonal Antibody
SKU:
CAB0327
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 113-261 of human CD40L (NP_000065.1).
Cell membrane, Secreted, Single-pass type II membrane protein.
Calculated MW:
29kDa
Observed MW:
25-30kDa/17kDa
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Purification Method:
Affinity purification
Gene ID:
959
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD40L antibody (CAB0327) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
