The CD4 Polyclonal Antibody (CAB0362) is a crucial tool for researchers studying the CD4 protein, a key player in the immune system's response to pathogens and foreign antigens. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for various applications, including Western blot analysis.CD4, also known as T-cell surface glycoprotein CD4, is essential for initiating immune responses by binding to major histocompatibility complex class II molecules on antigen-presenting cells. Its presence on helper T cells is crucial for coordinating immune responses and activating other immune cells.
The CD4 Polyclonal Antibody binds specifically to the CD4 molecule, allowing for precise detection and analysis in different cell types.The significance of CD4 in immune function and disease pathology makes it a valuable target for research in immunology, infectious diseases, and autoimmune disorders. By understanding the role of CD4 in immune regulation, researchers can develop targeted therapies for conditions like HIV/AIDS, inflammatory disorders, and cancer. The CD4 Polyclonal Antibody provides a reliable tool for investigating CD4 biology and its implications in various disease states.
Product Name:
CD4 Rabbit Polyclonal Antibody
SKU:
CAB0362
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
51kDa
Observed MW:
51kDa
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Purification Method:
Affinity purification
Gene ID:
920
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-937 cells, using CD4 Rabbit pAb (CAB0362) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
