The CD3E Polyclonal Antibody (CAB1753) is a valuable tool for researchers studying CD3E, a critical component of the T-cell receptor complex involved in immune responses. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the CD3E protein, this antibody enables precise detection and analysis in a variety of cell types, making it suitable for investigations in immunology and cancer research.CD3E is essential for T-cell activation and function, playing a key role in immune surveillance and response to antigens.
Dysregulation of CD3E expression or function has been implicated in autoimmune diseases, immunodeficiencies, and cancer. Therefore, thorough understanding of CD3E biology is crucial for the development of novel therapeutic strategies targeting T-cell mediated immune responses.In summary, the CD3E Polyclonal Antibody (CAB1753) is a reliable tool for researchers seeking to unravel the complexities of T-cell biology and its implications for immune-related disorders. Its high reactivity and specificity make it an indispensable asset for studies aiming to elucidate the role of CD3E in health and disease.
Product Name:
CD3E Rabbit Polyclonal Antibody
SKU:
CAB1753
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human CD3E (NP_000724.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
T3E; TCRE; IMD18; CD3epsilon; CD3E
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
23kDa
Observed MW:
23kDa
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Purification Method:
Affinity purification
Gene ID:
916
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using CD3E Rabbit pAb (CAB1753) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
