The CD33 Monoclonal Antibody (CAB22704) is a valuable tool for researchers studying CD33, a transmembrane receptor protein primarily expressed on cells of the myeloid lineage. This antibody, produced through hybridoma technology, is highly specific to human CD33 and is validated for use in various applications, including flow cytometry and immunohistochemistry.CD33 is known to play a role in regulating the activation and differentiation of myeloid cells, making it a key target for studies in immunology and hematological disorders. Research involving CD33 has focused on its implications in diseases such as acute myeloid leukemia (AML) and Alzheimer's disease, where abnormal expression or function of CD33 has been implicated.
By utilizing the CD33 Monoclonal Antibody (CAB22704), researchers can accurately detect and analyze CD33 expression in different cell types and tissues, providing valuable insights into the role of this protein in disease pathology and potential therapeutic interventions. This antibody is a valuable tool for advancing our understanding of CD33 biology and its implications in various disease states.
Product Name:
CD33 Monoclonal Antibody
SKU:
CAB22704
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-259 of human CD33 (NP_001763.3).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
40kDa
Observed MW:
70-80kDa
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Purification Method:
Affinity purification
Gene ID:
945
Clone Number:
ARC57811
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD33 antibody (CAB22704) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
