The CD320 Antigen Polyclonal Antibody (CAB8627) is a powerful tool for researchers studying CD320, a cell surface receptor involved in the regulation of vitamin B12 uptake and transport. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CD320 antigen, this antibody enables accurate detection and analysis of this important protein in a variety of cell types.CD320, also known as transcobalamin receptor, is essential for the cellular uptake of vitamin B12, a vital nutrient involved in various metabolic processes.
Dysregulation of CD320 has been implicated in a number of disorders related to vitamin B12 deficiency, making it a key target for research in areas such as nutrition, metabolism, and neurobiology. Investigating the function of CD320 with this antibody can provide valuable insights into the mechanisms underlying these conditions and pave the way for new therapeutic strategies.
Product Name:
CD320 Rabbit Polyclonal Antibody
SKU:
CAB8627
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 36-229 of human CD320 (NP_057663.1).
This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
51293
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of U-251MG, using CD320 Rabbit pAb (CAB8627) at 1:2500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
