The CD274 Polyclonal Antibody (CAB1645) is a valuable tool for studying CD274, also known as PDL1, a protein involved in immune regulation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to CD274, researchers can detect and analyze this important immune checkpoint protein in various cell types, making it an ideal choice for studies in immunology and cancer research.CD274 is a key player in the immune system, serving as a checkpoint molecule that helps regulate immune responses and prevent excessive inflammation. Its role in immune evasion has critical implications for diseases like cancer, where tumors can exploit CD274 to evade detection and destruction by the immune system.
By understanding the functions of CD274, researchers can uncover new therapeutic targets and develop innovative treatments for cancer and other immune-related disorders.In conclusion, the CD274 Polyclonal Antibody (CAB1645) is a high-quality reagent that enables precise and reliable detection of CD274 in research settings. Its specificity and sensitivity make it an indispensable tool for investigating the complex mechanisms of immune regulation and developing novel therapeutic strategies for a range of diseases.
Product Name:
PD-L1/CD274 Rabbit Polyclonal Antibody
SKU:
CAB1645
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 190-290 of human PD-L1/CD274 (NP_054862.1).
Cell membrane, Endomembrane system, Single-pass type I membrane protein.
Calculated MW:
33kDa
Observed MW:
40-50kDa
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
29126
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using PD-L1/CD274 Rabbit pAb (CAB1645) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.