The CD274 Monoclonal Antibody (CAB23922) is a powerful tool for researchers investigating the role of CD274, also known as programmed cell death 1 ligand 1 (PD-L1), in immune regulation and cancer therapy. This antibody, developed through monoclonal technology, exhibits high specificity and sensitivity to human CD274 protein, making it ideal for various experimental applications.CD274/PD-L1 is a critical immune checkpoint protein that plays a key role in suppressing immune responses and promoting immune tolerance. In cancer, CD274 expression on tumor cells allows them to evade the immune system's detection, making it a promising target for immunotherapy treatment strategies.
Research using the CD274 Monoclonal Antibody can provide valuable insights into the mechanisms of immune evasion in cancer and aid in the development of novel therapeutic approaches.The use of the CD274 Monoclonal Antibody in techniques such as immunohistochemistry, flow cytometry, and Western blotting allows for accurate detection and quantification of CD274 expression in various biological samples. Its reliability and specificity make it an essential tool for studies aimed at understanding the complex interactions between the immune system and cancer cells, paving the way for advancements in personalized cancer treatment strategies.
Product Name:
CD274 Monoclonal Antibody
SKU:
CAB23922
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-235 of mouse CD274(NP_068693.1).
Cell membrane, Endomembrane system, Single-pass type I membrane protein.
Calculated MW:
33kDa
Observed MW:
40-50kDa
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
60533
Clone Number:
ARC61782
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit mAb (CAB23922) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
