The CD19 Polyclonal Antibody (CAB2577) is a valuable tool for researchers studying CD19, a cell surface protein commonly found on B cells. This antibody, produced in rabbits, is highly specific and reliable for use in various research applications, including Western blotting and immunohistochemistry.CD19 is an essential component of the B cell receptor complex and plays a critical role in B cell development, activation, and differentiation. Dysregulation of CD19 expression has been linked to various B cell malignancies, making it a target of interest in cancer research.
The CD19 Polyclonal Antibody enables the detection and analysis of CD19 protein levels in different cell types, providing valuable insights into its function and potential therapeutic applications.With its high reactivity and specificity, the CD19 Polyclonal Antibody is an excellent choice for researchers investigating B cell biology, immunology, and cancer. By understanding the role of CD19 in normal and disease states, scientists can advance their knowledge of immune responses and potentially develop targeted therapies for B cell-related disorders.
Product Name:
CD19 Rabbit Polyclonal Antibody
SKU:
CAB2577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 457-556 of human CD19 (NP_001761.3).
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
930
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Rat lung, using CD19 Rabbit pAb (CAB2577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
