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CD19 Rabbit Polyclonal Antibody (CAB2577)

SKU:
CAB2577
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-CD19 Antibody CAB2577
  • Immunology Antibodies 2 Anti-CD19 Antibody CAB2577
  • Immunology Antibodies 2 Anti-CD19 Antibody CAB2577
  • Immunology Antibodies 2 Anti-CD19 Antibody CAB2577
  • Immunology Antibodies 2 Anti-CD19 Antibody CAB2577
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CD19 Rabbit Polyclonal Antibody
SKU:CAB2577
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 457-556 of human CD19 (NP_001761.3).
Sequence:NAES YENE DEEL TQPV ARTM DFLS PHGS AWDP SREA TSLG SQSY EDMR GILY AAPQ LRSI RGQP GPNH EEDA DSYE NMDN PDGP DPAW GGGG RMGT WSTR
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:B4; CVID3; CD19
Positive Sample:Rat lung
Conjugate:Unconjugated
Cellular Localization:Membrane, Single-pass type I membrane protein.
Calculated MW:61kDa
Observed MW:95kDa

This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification Method:Affinity purification
Gene ID:930
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of Rat lung, using CD19 Rabbit pAb (CAB2577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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