The CD160 Antigen Polyclonal Antibody (CAB8189) is a specialized tool for researchers studying CD160, a cell surface antigen with implications in immune regulation and cell signaling. This antibody, produced through rabbit immunization, offers high reactivity with human samples and is validated for Western blot applications. By binding specifically to the CD160 antigen, it enables precise detection and analysis in various cell types, making it a valuable asset for investigations in immunology, cancer research, and beyond.CD160, also known as Natural Killer Cell Receptor BY55, is known to function as a co-stimulatory molecule in T-cell activation and has been linked to both innate and adaptive immune responses.
Its diverse role in immune regulation suggests potential relevance in various diseases and conditions, including cancer, infectious diseases, and autoimmune disorders. By elucidating the mechanisms underlying CD160 activity, researchers can advance their understanding of immune function and explore novel therapeutic strategies in related fields.
Product Name:
CD160 Rabbit Polyclonal Antibody
SKU:
CAB8189
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human CD160 (NP_008984.1).
Jurkat,HT-29,U-937,Raji,Mouse spleen,Mouse small intestine,Mouse thymus,Rat thymus
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, GPI-anchor, Lipid-anchor.
Calculated MW:
20kDa
Observed MW:
20kDa
CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules.
Purification Method:
Affinity purification
Gene ID:
11126
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD160 antibody (CAB8189) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
