The CD146 Polyclonal Antibody is a valuable tool for researchers studying CD146, a cell surface adhesion molecule involved in various physiological processes such as cell migration, angiogenesis, and tumor metastasis. This antibody, generated in rabbits, exhibits high specificity towards human samples and is suitable for use in Western blot assays. By targeting the CD146 protein, researchers can easily detect and analyze its expression in different cell types, making it an ideal tool for investigations in cell biology, oncology, and vascular biology.
CD146, also known as Mel-CAM or MUC18, plays a crucial role in promoting cell adhesion and migration, making it a key player in processes like wound healing and tumor progression. Its involvement in angiogenesis also makes it a potential target for anti-cancer therapies aimed at disrupting tumor vascularization. By studying the functions of CD146, researchers can gain valuable insights into how this molecule contributes to disease progression and develop novel treatment strategies targeting its activity.
Product Name:
CD146 Polyclonal Antibody
SKU:
CAB23669
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant protein of mouse CD146.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CD146; CD149; Muc18; s-endo; 1-gicerin; s-gicerin
Positive Sample:
HeLa,MCF7,Mouse heart
Conjugate:
Unconjugated
Calculated MW:
71kDa
Observed MW:
120kDa
Predicted to be involved in angiogenesis and positive regulation of cell migration. Predicted to act upstream of or within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Predicted to be located in external side of plasma membrane; extracellular space; and nucleus. Predicted to be active in plasma membrane. Is expressed in several structures, including adipose tissue; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human MCAM (melanoma cell adhesion molecule).
Purification Method:
Affinity purification
Gene ID:
84004
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD146 Rabbit pAb (CAB23669) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
