The CD144 Polyclonal Antibody is a powerful tool for researchers studying CD144, a cell surface molecule known for its involvement in vascular integrity and angiogenesis. This antibody, produced through the immunization of rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CD144 protein, this antibody allows for the detection and analysis of CD144 expression in various cell types, making it a valuable asset in studies related to vascular biology and cancer research.
CD144, also referred to as VE-cadherin, plays a crucial role in maintaining endothelial barrier function and promoting angiogenesis, processes essential for normal vascular development and function. Its involvement in vascular stability and permeability regulation has made it a target of interest in research on diseases such as cancer, ischemic disorders, and inflammatory conditions. Understanding the functions of CD144 is key to developing strategies for manipulating vascular integrity and angiogenesis in a variety of pathological contexts.
Product Name:
CD144 Polyclonal Antibody
SKU:
CAB24490
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 46-592 of mouse CD144 (NP_033998.2).
This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8.
Purification Method:
Affinity purification
Gene ID:
12562
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Mouse lung, using CD144 Rabbit pAb (CAB24490) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
