The CD14 Antibody (CAB5737) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the CD14 protein, this antibody allows for the detection and analysis of CD14 in a variety of cell types, making it a valuable resource for investigations in immunology and infectious disease research.CD14 is a co-receptor for bacterial lipopolysaccharide (LPS) and plays a critical role in the recognition and response to microbial pathogens. Its involvement in the inflammatory response makes it a target for understanding the mechanisms of sepsis, a life-threatening condition caused by the body's overwhelming response to infection.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD14 Antibody
SKU:
CAB5737
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
929
RRID
AB_2766493
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of lysates from RAW264.7 cells, using CD14 Rabbit pAb (CAB5737) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of RAW264.7 cells using CD14 Rabbit pAb (CAB5737) at dilution of 1:100. Blue: DAPI for nuclear staining.
