The CD11b Polyclonal Antibody is a valuable tool for researchers studying the CD11b cell surface protein, which plays a key role in immune responses and inflammation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.CD11b, also known as integrin alpha M, is a crucial component of the Mac-1 integrin receptor expressed on certain immune cell types, including macrophages, monocytes, and neutrophils. Its involvement in cell adhesion, phagocytosis, and migration makes it a central player in the immune response to infections and inflammatory conditions.
By targeting the CD11b protein, researchers can gain insights into immune cell function and interactions in various disease contexts, including infectious diseases, autoimmune disorders, and inflammatory conditions. This antibody enables the detection and analysis of CD11b expression in different cell types, providing valuable information for immunological and cancer research studies. Understanding the role of CD11b in immune regulation is essential for developing targeted therapies to modulate immune responses effectively.
Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M).
Purification Method:
Affinity purification
Gene ID:
16409
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD11b Rabbit pAb (CAB23254) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
