The CD117/c-kit Monoclonal Antibody (CAB22750) is a cutting-edge tool for researchers investigating the CD117/c-kit protein, a crucial marker in cell biology and cancer research. This antibody, produced using advanced monoclonal technology, specifically targets the CD117/c-kit protein in human samples and is validated for use in various applications, including immunohistochemistry and flow cytometry.CD117/c-kit, a receptor tyrosine kinase, plays a key role in cell growth, differentiation, and survival, making it a valuable target for studying various cellular processes and identifying potential therapeutic targets in cancer and other diseases. By targeting the CD117/c-kit protein, researchers can further explore its role in signaling pathways and its potential as a biomarker for various cancers, particularly gastrointestinal stromal tumors (GISTs) where CD117/c-kit mutations are common.
With its high specificity and reliability, the CD117/c-kit Monoclonal Antibody (CAB22750) is an essential tool for scientists conducting research in cell biology, cancer biology, and drug development. Its ability to precisely detect and target the CD117/c-kit protein opens up new possibilities for understanding the mechanisms underlying disease and developing targeted therapies in the field of oncology.
Product Name:
CD117/c-Kit Monoclonal Antibody
SKU:
CAB22750
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-520 of human c-Kit (NP_000213.1)
Cell membrane, Cytoplasm, Single-pass type I membrane protein.
Calculated MW:
110kDa
Observed MW:
150kDa
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3815
Clone Number:
ARC56007
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HEL cells, using CD117/c-Kit Rabbit mAb (CAB22750) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
