The CCT4 Polyclonal Antibody (CAB6548) is specially designed for research involving CCT4, a protein that plays a crucial role in maintaining cell viability and protein folding. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the CCT4 protein, this antibody allows for the detection and analysis of CCT4 levels in various cell types, making it an invaluable tool for studies in molecular biology and cell biology.
CCT4, a subunit of the chaperonin-containing TCP1 complex, is essential for the proper folding of proteins and the prevention of protein aggregation. Dysregulation of CCT4 has been implicated in various diseases, including neurodegenerative disorders and cancer, highlighting its importance in cellular homeostasis. By studying CCT4 expression and function, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
CCT4 Rabbit Polyclonal Antibody
SKU:
CAB6548
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).
The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).
Purification Method:
Affinity purification
Gene ID:
10575
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CCT4 antibody (CAB6548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
