The Cyclin H Polyclonal Antibody (CAB0995) is a valuable tool for researchers studying Cyclin H, a protein involved in cell cycle regulation and DNA repair processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is specifically validated for use in Western blot applications.Cyclin H is a key player in cell cycle progression, functioning as a regulatory subunit of the CDK7 kinase complex. It is essential for cell division and DNA replication, making it a crucial target for studies in cancer biology and developmental biology. With the ability to detect and analyze Cyclin H protein levels in various cell types, this antibody is well-suited for immunology and cancer research applications.
Furthermore, Cyclin H has been implicated in the pathogenesis of several diseases, including cancer and neurodegenerative disorders. By investigating the expression and function of Cyclin H using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for treatment.Overall, the Cyclin H Polyclonal Antibody (CAB0995) offers researchers a reliable tool for studying the role of Cyclin H in cell cycle regulation, DNA repair, and disease pathogenesis, ultimately advancing our understanding of critical biological processes and potential therapeutic interventions.
Product Name:
Cyclin H Rabbit Polyclonal Antibody
SKU:
CAB0995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human Cyclin H (NP_001230.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CAK; p34; p37; CycH; Cyclin H
Positive Sample:
Jurkat,K-562,SW620,HT-29,MCF7,Jurkat,K-562,SW620
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
36kDa/
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
902
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin H Rabbit pAb (CAB0995) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
