The CCNF Polyclonal Antibody (PACO14312) is a valuable tool for researchers studying CCNF, a key protein involved in cell cycle regulation and cancer development. This antibody, raised in rabbits, is highly specific to human CCNF samples and has been validated for use in Western blot applications. It binds to the CCNF protein, allowing for accurate detection and analysis in various cell types.CCNF, also known as Cyclin F, is a crucial regulator of the cell cycle, playing a role in cell division and DNA replication. Abnormalities in CCNF expression have been linked to various types of cancer, making it an important target for cancer research.
By studying the function of CCNF, researchers can gain insights into cancer progression and potentially identify new therapeutic targets for cancer treatment.Overall, the CCNF Polyclonal Antibody is a valuable tool for researchers interested in studying the role of CCNF in cancer development and cell cycle regulation. Its high specificity and sensitivity make it ideal for use in a variety of research applications, including Western blotting and immunohistochemistry.
Gel: 10+12%SDS-PAGE, Lysate: 40 μg, Lane: Human lymphoma tissue, Primary antibody: PACO14312(CCNF Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO14312(CCNF Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid, motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.
Synonyms:
cyclin F
UniProt Protein Function:
CCNF: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Belongs to the cyclin family. Cyclin AB subfamily.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 16p13.3Cellular Component: centriole; SCF ubiquitin ligase complex; nucleusMolecular Function: protein bindingBiological Process: mitosis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; re-entry into mitotic cell cycle; cell division; protein ubiquitination; placenta development
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008]
