The CCNF Polyclonal Antibody (PACO00654) is a valuable tool for researchers studying the CCNF protein, a crucial regulator of cell cycle progression and protein degradation. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is specifically designed for use in Western blot applications. By targeting the CCNF protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component for studies in oncology and cell biology research.
CCNF, also known as cyclin F, plays a pivotal role in controlling cell division and protein turnover, making it a key player in maintaining cellular homeostasis. Dysregulation of CCNF has been associated with various diseases, including cancer and neurological disorders, highlighting its importance as a potential therapeutic target. Unraveling the functions of CCNF is essential for developing targeted treatments that can manipulate cell cycle progression and protein degradation pathways in disease contexts.
Antibody Name:
CCNF Antibody
Antibody SKU:
PACO00654
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the C-terminal region of human Cyclin F.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
CCNF; FBX1; FBXO1; Cyclin-F; F-box only protein 1
UniProt Protein Function:
CCNF: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Belongs to the cyclin family. Cyclin AB subfamily.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 16p13.3Cellular Component: centriole; SCF ubiquitin ligase complex; nucleusMolecular Function: protein bindingBiological Process: mitosis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; re-entry into mitotic cell cycle; cell division; protein ubiquitination; placenta development
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008]
