The CCNE1 Polyclonal Antibody (CAB14225) is a valuable tool for researchers studying the CCNE1 protein, a key regulator of cell cycle progression and DNA replication. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the CCNE1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for investigations in cancer biology and cell cycle regulation.
CCNE1 is a crucial factor in regulating the cell cycle and ensuring accurate DNA replication, making it a target of interest in cancer research and therapeutic development. Dysregulation of CCNE1 has been implicated in various types of cancer, highlighting its importance as a potential biomarker and therapeutic target. By studying the role of CCNE1 in cell cycle control, researchers can gain valuable insights into cancer progression and identify novel strategies for intervention.
Product Name:
Cyclin E1 Rabbit Polyclonal Antibody
SKU:
CAB14225
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 (NP_001229.1).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Purification Method:
Affinity purification
Gene ID:
898
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin E1 Rabbit pAb (CAB14225) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
