The CCND2 Polyclonal Antibody (CAB1773) is a key tool for researchers studying CCND2, a protein involved in cell cycle regulation and cell proliferation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CCND2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an invaluable resource for studies in cancer biology and cell biology.
CCND2, also known as cyclin D2, plays a crucial role in regulating the progression of the cell cycle and promoting cell division. Dysregulation of CCND2 has been implicated in various cancers and other diseases related to uncontrolled cell growth. By studying the function and expression of CCND2 using this antibody, researchers can gain insights into the mechanisms underlying these pathological conditions and potentially identify novel targets for therapeutic interventions.
Product Name:
Cyclin D2 Rabbit Polyclonal Antibody
SKU:
CAB1773
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human Cyclin D2 (NP_001750.1).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).
Purification Method:
Affinity purification
Gene ID:
894
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cyclin D2 antibody (CAB1773) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
