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Cyclin D1 Rabbit Polyclonal Antibody (CAB2708)

SKU:
CAB2708
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cyclin D1 Rabbit Polyclonal Antibody
SKU:CAB2708
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-295 of human Cyclin D1 (NP_444284.1).
Sequence:PSMV AAGS VVAA VQGL NLRS PNNF LSYY RLTR FLSR VIKC DPDC LRAC QEQI EALL ESSL RQAQ QNMD PKAA EEEE EEEE EVDL ACTP TDVR DVDI
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:BCL1; PRAD1; U21B31; D11S287E; Cyclin D1
Positive Sample:SH-SY5Y,MCF7,NIH/3T3,Mouse heart
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Membrane, Nucleus.
Calculated MW:34kDa
Observed MW:34kDa

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Purification Method:Affinity purification
Gene ID:595
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Cyclin D1 Rabbit pAb (CAB2708) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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